herring sperm dna substrates (Thermo Fisher)
Structured Review

Herring Sperm Dna Substrates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/herring+sperm+dna/DNA/pmc12706800-140-14-22
Average 99 stars, based on 1 article reviews
Images
1) Product Images from "Establishment of the first Chinese national standard for recombinant nuclease used as a process material in pharmaceutical manufacturing"
Article Title: Establishment of the first Chinese national standard for recombinant nuclease used as a process material in pharmaceutical manufacturing
Journal: Frontiers in Bioengineering and Biotechnology
doi: 10.3389/fbioe.2025.1695176
Figure Legend Snippet: Establishment of substrate quality standards for the activity determination method. (A) Nucleic acid electrophoresis results of DNA substrates from three manufacturers. Lanes 1–3: DNA substrate from Solarbio; Lanes 4–6: DNA substrate from Invitrogen; Lanes 7–9: DNA substrate from Promega; M: marker. (B) Linear equations fitted by plotting ΔOD 260 against time for reactions between RSN and DNA substrates from three manufacturers, n = 3. (C) Nucleic acid electrophoresis results of reactions between RSN and DNA substrate from Invitrogen at different times. Lanes 1–6: 5, 15, 30, 45, 60, and 90 min, respectively; Lane 7: unreacted DNA substrate control. (D) Nucleic acid electrophoresis results of reactions between RSN and DNA substrate from Solarbio at different times. Lanes 1–6: 5, 15, 30, 45, 60, and 90 min, respectively; Lane 7: unreacted control.
Techniques Used: Activity Assay, Nucleic Acid Electrophoresis, Marker, Control
Related Articles
Saline:Article Title: Sensitive, high-throughput, metabolic analysis by molecular sensors on the membrane surface of mother yeast cells. Article Snippet: Alexa Fluor 488-Concanavalin A (ConA) was obtained from Biotium, USA. .. Dulbecco’s Phosphate-Buffered Saline (DPBS), tris buffer (1M, pH 7.0 or 8.0), Article Title: Niemann-Pick type C2 protein PpseNPC2-1 binding volatile nerolidol mediates prey localization of the pond wolf spider Pardosa pseudoannulata. Article Snippet: As a key natural enemy of common agricultural pests, the pond wolf spider Pardosa pseudoannulata preys on planthoppers, leafhoppers and other pests.. Chemoreception is essential for P. pseudoannulata in locating prey and searching for mates.. Although our previous work identified Niemann-Pick type C2 (NPC2) proteins as olfactoryrelated in P. pseudoannulata, the molecular mechanisms underlying prey detection remain unclear. Article Title: Sensitive, high-throughput, metabolic analysis by molecular sensors on the membrane surface of mother yeast cells Article Snippet: Alexa Fluor 488-Concanavalin A (ConA) was obtained from Biotium, USA. .. Dulbecco’s Phosphate-Buffered Saline (DPBS), tris buffer (1 M, pH 7.0 or 8.0), Hybridization:Article Title: PINK1 deficiency permits the development of Lewy body dementia with coexisting Aβ pathology. Article Snippet: 2.3.6 RNA in situ hybridization and immunofluorescence Brain sections were first treated with 2 μg/mL Proteinase K (Invitrogen, 4333793) for 10 minutes, followed by a 10 minute incubation in 0.1 M Triethanolamine (TEA; Sangon Biotech, A600970). .. Sections were placed in a humidified chamber and pre-hybridized for 1 hour at room temperature with a hybridization solution containing 50% formamide (Invitrogen, 15515-026), 5×SSC (Invitrogen, 15557044), 5×Denhardt’s solution (Invitrogen, 750018), 0.25 mg/mL yeast RNA (Biotechnology, R0038), and 0.5 mg/mL Article Title: PINK1 deficiency permits the development of Lewy body dementia with coexisting Aβ pathology Article Snippet: Brain sections were first treated with 2 μg/mL Proteinase K (Invitrogen, 4333793) for 10 minutes, followed by a 10 minute incubation in 0.1 M Triethanolamine (TEA; Sangon Biotech, A600970). .. Sections were placed in a humidified chamber and pre‐hybridized for 1 hour at room temperature with a hybridization solution containing 50% formamide (Invitrogen, 15515‐026), 5×SSC (Invitrogen, 15557044), 5×Denhardt's solution (Invitrogen, 750018), 0.25 mg/mL yeast RNA (Biotechnology, R0038), and 0.5 mg/mL Article Title: Niemann-Pick type C2 protein PpseNPC2-1 binding volatile nerolidol mediates prey localization of the pond wolf spider Pardosa pseudoannulata. Article Snippet: As a key natural enemy of common agricultural pests, the pond wolf spider Pardosa pseudoannulata preys on planthoppers, leafhoppers and other pests.. Chemoreception is essential for P. pseudoannulata in locating prey and searching for mates.. Although our previous work identified Niemann-Pick type C2 (NPC2) proteins as olfactoryrelated in P. pseudoannulata, the molecular mechanisms underlying prey detection remain unclear. Article Title: PUS10-induced tRNA fragmentation impacts retrotransposon-driven inflammation. Article Snippet: The membrane was dried and pre-hybridized at 55◦C for 30 min in PerfectHyb Plus Hybridization Buffer (Sigma) and 0.1 mg/mL herring sperm DNA (Thermo Fisher). .. Hybridization was performed in fresh hybridization buffer containing 1 × 106 cpm/ml of 32P-labeled tRNA-GlyGCC probe and 0.1 mg/mL Incubation:Article Title: Niemann-Pick type C2 protein PpseNPC2-1 binding volatile nerolidol mediates prey localization of the pond wolf spider Pardosa pseudoannulata. Article Snippet: As a key natural enemy of common agricultural pests, the pond wolf spider Pardosa pseudoannulata preys on planthoppers, leafhoppers and other pests.. Chemoreception is essential for P. pseudoannulata in locating prey and searching for mates.. Although our previous work identified Niemann-Pick type C2 (NPC2) proteins as olfactoryrelated in P. pseudoannulata, the molecular mechanisms underlying prey detection remain unclear. other:Article Title: PUS10-induced tRNA fragmentation impacts retrotransposon-driven inflammation. Article Snippet: Subsequently, the membrane was washed once in low stringency buffer (2× SSC, 0.1% SDS) at room temperature for 5 min and twice in high stringency buffer (0.5× SSC, 0.1% SDS) at 42◦C for 20 min. Quantification was performed overnight using a phosphorimager (Fuji film FLA3000). |